Meridian Bioscience Glycerol-Free T4 DNA Polymerase (HC) - 1000 Units

Glycerol-Free T4 DNA Polymerase (HC) is a template dependent DNA polymerase that catalyses 5'-3' synthesis from primed single-stranded DNA and possess a 3´➔ 5´ exonuclease activity but lacks a 5´➔ 3´ exonuclease activity. Glycerol-Free T4 DNA Polymerase (HC) can be used for generating blunt ends on any duplex DNA molecule and for labelling DNA by replacement synthesis.

Glycerol-Free T4 DNA Polymerase (HC) is supplied at 10 U/µL and catalyses the addition of nucleotides to the 3′ end of a DNA strand, extending it in the 5′ to 3′ direction. It also possesses 3′ to 5′ exonuclease activity, allowing it to remove nucleotides from the 3′ end of DNA strands. This activity is useful in various DNA manipulation techniques, such as DNA sequencing, site-directed mutagenesis, and DNA repair studies. In addition, it has 5′ to 3′ exonuclease activity, enabling it to remove nucleotides from the 5′ end of DNA molecules.

It is used in next-generation sequencing (NGS) library preparation, where it can be used separately or as part of an End-Repair Mix, where it can blunt DNA ends of double-stranded DNA fragments for subsequent ligation of adaptors by T4 DNA Ligase.

Glycerol-Free T4 DNA Polymerase (HC) is in a glycerol-free storage buffer and is supplied with a reaction buffer and has been optimized to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilisation. This gives you the flexibility to confidently store mixtures at ambient temperature or produce diagnostic assays that rely on miniaturised reaction components, such as point-of-care NGS.

• Blunting of DNA ends by "fill in" or "polish" the ends of double-stranded DNA fragments, for cloning or generating NGS libraries
• Oligonucleotide-directed site-directed mutagenesis
• Sanger sequencing, to remove fluorescent dye-labelled nucleotides as they are incorporated into the growing DNA strand
• Glycerol-free, for lyophilisation and ambient temperature storage